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KMID : 0613820130230101223
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Journal of Life Science
2013 Volume.23 No. 10 p.1223 ~ p.1229
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Effect of Ursolic Acid on the Development of Mouse Embryonic Stem Cells under Hypoxia
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Han Gi-Yeon
Park Jae-Hong
Oh Keon-Bong
Lee Sei-Jung
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Abstract
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Ursolic acid (UA) a bio-active ingredient found in a variety of fruits and vegetables, and it has potent antioxidant activity. However, the role of UA in mouse embryonic stem (ES) cells is poorly understood. This study investigated the functional role of UA in regulating the development of mouse ES cells under hypoxia. Hypoxia did not exert a significant effect on the undifferentiated state of mouse ES cells. However, it induced reactive oxygen species (ROS) generation and increased the level of lactate dehydrogenase (LDH) production at 48 h of hypoxic exposure. Conversely, oxidative stress induced by hypoxia was significantly inhibited by UA (30μM) pretreatment. Hypoxia significantly decreased cell survival and the level of [³H] thymidine incorporation, both of which recovered following pretreatment of UA. In addition, UA decreased the apoptotic effect of hypoxia by attenuating caspase-3 cleavage or by recovering cellular inhibition of the apoptotic protein (cIAP)-2 and Bcl-2 expression. We further found that UA decreased senescence-associated beta-galactosidase activity. We suggest that UA is a natural antioxidant and one of the functional modulators of hypoxia-induced survival, apoptosis, proliferation, and aging in mouse ES cells.
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KEYWORD
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Apoptosis, mouse embryonic stem (ES) cells, proliferation, senescence, survival
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